The Portuguese coach says his side is in no danger of underestimating Standard in its playoff second leg and is confident Hulk will be fit to play.
The Russian giant takes a 1-0 first-leg lead against Belgian side Standard Liege back to St Petersburg in the second leg of their playoff tie Tuesday.
Villas-Boas insists there will be no danger of his side underestimating its opponents as it aims to clinch a return to Europe's top competition proper.
"All the details matter at this level, that's the nature of the Champions League," the former Chelsea and Tottenham boss told reporters. "I don't think that there's any reason to talk about underestimating or a lack of motivation because we both want to move forward.
"This is the last chance, no one has any excuses left. Our emotions will be about the same probably. We just hope to make it through."
Brazil international Hulk is a doubt for the clash after picking up a knock against Amkar at the weekend, but Villas-Boas is confident the forward will be ready to play a part.
"We'll see," he said of the former Porto star. "Everyone saw that he was substituted in the game against Amkar, but we are confident that he will play. We'll see how the situation develops."