The Blancos attacker had to leave training because of an illness and will sit out the Santiago Bernabeu showdown with BVB.
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"Di Maria had to leave Real Madrid training today [Wednesday] due to gastroenteritis," a club statement read. "The Argentine will be out of the match against Borussia Dortmund in the first leg of the Champions League quarterfinals.
"His position will be taken by Jose Rodriguez."
The 26-year-old Di Maria joins left back Marcelo on the sidelines for the Santiago Bernabeu clash.