City's striker is likely to be out of action for a month following a hamstring injury sustained at Tottenham and is set to miss out on the Champion League clash against Barcelona.
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City, which returned to the top of the Premier League in midweek, is due to face Tata Martino’s side in the first leg of their Champions League last 16 tie at the Etihad Stadium on Feb. 18, with the return fixture at Camp Nou scheduled for March 12.
Manchester City manager Manuel Pellegrini said: "[Samir] Nasri continues treatment. Javi Garcia and Kun Aguero are injured. [The] doctor has seen Aguero and he will be out for a month.
"It will be difficult because he is a top player for us but we have the players to replace him. He has just come back from injury and Alvaro Negredo, Stevan Jovetic and Edin Dzeko can replace him."