Both sides have already faced each other twice in this season's competition, with BVB winning 2-1 at home in the group stages, before drawing 2-2 at the Santiago Bernabeu.
And with Klopp enjoying the superior head-to-head record between the pair on the back of those results, Mourinho has questioned why the German coach has opted to discuss their next European encounter so much in the buildup to the game.
"I have said a little bit about Dortmund and that's enough. Since the day of the draw, Klopp talks every day," he told reporters. "I will talk about Dortmund this Tuesday. We have been working on this match for the past two weeks."
Klopp recently attempted some possible mind games of his own by suggesting that Mourinho's trip to watch Dortmund defeat Greuther Furth 6-1 last weekend was a pointless exercise.
“Mourinho will return home with reports that will be useless to him in two weeks time," he added. “I don't know if Madrid will be afraid of us after what happened in the group stage.
"He left after the 5-0 and didn't see our weaknesses. But if he wanted to see whether [Mario] Gotze can play on the left, he can call me."
The first leg takes place in Dortmund on Wednesday, with the return fixture at the Bernabeu on April 30.