Geoff Cameron has withdrawn from the U.S. roster set to face the Czech Republic on Wednesday with a previously identified side strain, U.S. Soccer announced Sunday.
The federation said the decision was made after "further consulation" with Cameron's club, Stoke City. The 29-year-old will not be replaced on the roster.
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Cameron suffered the injury in Stoke's 3-0 win over Portsmouth in the Capital One Cup on Wednesday, exiting in the 34th minute. Stoke manager Mark Hughes then said Cameron would be "out for a couple of weeks," but the player was still named to U.S. coach Jurgen Klinsmann's squad Thursday.
John Brooks, Timmy Chandler, Greg Garza, Fabian Johnson, Michael Orozco and Tim Ream are the remaining defenders on the U.S. team traveling to Prague.
The injury could also play a role in any potential move by Cameron before the transfer window closes Monday. Earlier this month, Goal USA reported that the player had requested a transfer.