Team GB was beaten by a Mexico Under-23 side in a behind closed doors friendly in Marbella on Sunday.
Chivas forward Marco Fabian scored the only goal of a game played over three 30-minute thirds in the 28th minute, as both sides continued their preparations for the 2012 Olympics in London.
All three of Team GB's senior players - Ryan Giggs, Craig Bellamy and Micah Richards - featured in the friendly, in a team which also included Manchester United's Tom Cleverley, Arsenal midfielder Aaron Ramsey and Chelsea left back Ryan Bertrand.
There was also a start for Jack Butland - England's third-choice goalkeeper at Euro 2012 - and Tottenham's Danny Rose, along with Under-21 internationals Marvin Sordell, Craig Dawson and Jack Cork.
After the game, Rose tweeted: "Just played Mexico. Was great to get my first 60 minutes under my belt ... Lost 1-0 though."