The Swiss shot-stopper is expected to miss eight to ten weeks.
Frei will undergo an MRI next week to determine the extent of his injury and whether surgery is required.
In Frei's place, Milos Kocic will step in as the team's number one goalkeeper. The 26-year-old Serbian made seven starts last season, and impressed in CONCACAF Champions League action last week, leading TFC to an upset quarterfinal victory over the LA Galaxy.
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