The north London outfit is already assured of winning Group K, and Andre Villas-Boas is expected to rest a number of key players ahead of Sunday's clash with Liverpool
Andre Villas-Boas's side have already guaranteed top spot with five consecutive triumphs but will be eager to emerge from the group with an unblemished record.
Spurs are one of the favourites to win the competition and have lived up to that billing in a campaign that has seen them concede just one goal.
Anzhi, which reached the round of 16 last season, could not find a way past Tottenham's defense when the two met in Russia in October.
First half goals from Jermain Defoe and Nacer Chadli secured the visitors' triumph on that occasion, but a run of seven points from three subsequent games has seen Anzhi qualify anyway.
Gadzhi Gadzhiyev's men are in deep trouble on the domestic front, languishing at the foot of the Russian Premier League table without a win all season.
Finding the net has also been a struggle for Anzhi, which has not scored in seven top-flight matches.
Tottenham has remedied its own troubles in front of goal recently, scoring eight in its last four across all competitions.
Defoe has been Villas-Boas's most reliable provider of goals in Europa League contests. His seven strikes in the tournament this term, including two in the qualifying phase, have elevated the England international to the top of the club's all-time scoring list in European matches.
Back-to-back league victories, which followed a 2-2 home draw against defending champions Manchester United, have lifted the London club up to sixth in the Premier League, just three points shy of second-placed Liverpool.
With European progress assured and a fixture against Brendan Rodgers's side coming up on Sunday, Villas-Boas is likely to make changes to his starting lineup. The likes of goalkeeper Brad Friedel, defender Zeki Fryers and midfielder Erik Lamela could all be drafted in.
Gadzhiyev does not have the luxury of a large squad so will need to rely on the same group of players who have defied their lowly league status to book a place in the next round.