Manuel Pellegrini's men were beaten 1-0 by the Blues when they met at the Etihad Stadium on February 3 but come into their FA Cup rematch fresh after a midweek postponement.
The game marks the second meeting between the two sides at the Etihad Stadium in the space of 12 days, with the Blues having come out on top in the previous clash. Fullback Branislav Ivanovic scored the winner on Feb. 3 as City's 20-match unbeaten run across all competitions came to an abrupt halt in a 1-0 win for Jose Mourinho's men.
As well as holding home advantage, however, City heads into Saturday's encounter fresher than its opponent after the Premier League clash with Sunderland was postponed on Wednesday due to adverse weather conditions.
Sergio Aguero (hamstring), midfielder Fernandinho (groin) and center back Matija Nastasic (knee) are all set to miss out. Meanwhile, midfield pair Samir Nasri and Javi Garcia have recently returned to training following knee injuries but their involvement looks doubtful.
Chelsea had been riding the crest of a wave as the club followed its win at City with a 3-0 home triumph over Newcastle. Mourinho's side suffered a slight setback on Tuesday, however, when it was held to 1-1 draw at West Brom, with Victor Anichebe canceling out Ivanovic's opener.
The Blues remain at the top of the Premier League table, though City now has a game in hand and a far superior goal difference.
Chelsea has enjoyed a period of success in the world's oldest cup competition in recent times, lifting the trophy on four of the last seven occasions with the last triumph coming in 2012 thanks to a 2-1 win over Liverpool in the final.
Captain John Terry could come back into contention for Mourinho following a muscular issue, while Gary Cahill is struggling with a calf problem.