Walter Mazzarri's men scraped past Sassuolo last weekend to record their first win of 2014 but will be up against the Viola who reached the Coppa Italia final midweek.
Vincenzo Montella's men reached the cup final after a 2-0 win over Udinese on Tuesday completed a 3-2 aggregate victory.
A victory over Inter at the Stadio Artemio Franchi would be another huge boost for Fiorentina, which is fourth in the table.
Walter Mazzarri's Inter side is fifth – eight points behind Fiorentina – and a loss would be a big blow to the club's European qualification hopes.
The loss of Giuseppe Rossi to a knee injury has hurt Montella's men, with the Italian striker having scored 14 goals in the league prior to going down. The Viola are to assess defender Stefan Savic, while midfielder Massimo Ambrosini is out.
Inter broke a six-match winless run in all competitions last time out, edging Sassuolo 1-0 thanks to Walter Samuel's goal, though the club is still without an away win in 2014.
Mazzarri has been under pressure and criticized for not using his younger players more often, including the likes of Mateo Kovacic and Mauro Icardi. However, Mazzarri will not have Esteban Cambiasso and Ricky Alvarez at his disposal for the trip to Florence.
Inter has a good record against Fiorentina, having tasted defeat just once in the previous 17 meetings between the teams.