The visitors have already assured themselves of qualification for next year's World Cup but Morten Olsen's men still have it all to do.
Morten Olsen's side is currently third in Group B on 12 points from eight matches, one point behind Bulgaria. Italy has already qualified for the 2014 showpiece with 20 points.
The Danes are looking to qualify for their fifth World Cup, but will have to better Bulgaria's results in the remaining two games to reach the playoffs. Facing Italy is not what Olsen would have wanted with so much on the line for his players as defeat on Friday and a Bulgaria win against Armenia would end Denmark's dreams of finishing second.
Denmark has only beaten Italy once in their last seven meetings and fell to a 3-1 loss when the sides last faced each other in Milan in October 2012.
Olsen, though, has plenty of talent available at his disposal as the likes of Viktor Fischer and Christian Eriksen have both impressed at club level in recent weeks.
Fischer has established himself at Ajax this season and shone in the club's 1-1 draw with Milan in the Champions League last month, while Eriksen has hit the ground running in the Premier League since his arrival at Tottenham from Ajax at the end of August.
Much will depend on the duo maintaining their recent form, while injuries within the Italy squad could give Denmark further hope.
Mario Balotelli was absent from training on Wednesday with an unknown injury problem, while Marco Verratti and Lorenzo Insigne are both minor doubts after missing sessions earlier in the week.
Italy was the second nation after the Netherlands to qualify from Europe for the World Cup, and coach Cesare Prandelli is expected to experiment with his lineup in the final two matches of the group.
Prandelli's side is unbeaten in the group so far, winning six games and drawing the other two, and will want to extend that run despite already securing the top spot after a 2-1 victory over Czech Republic last month.