The Danes have sprung the first surprise of the tournament, with victory against the Dutch belittling their billing as outsiders and throwing Group B wide open.
Despite fashioning a slew of chances almost straight from kickoff, the tournament favorite was extremely poor in front of goal, and the team's profligacy was punished by a fine finish from Michael Krohn Dehli after 24 minutes.
The Dutch were similarly wasteful during the second half, their performance epitomized by the laboring Robin van Persie, who showed none of the killer instinct from his stellar 37-goal season with Arsenal.
The race for qualification from Group B is now wide open, after a stunning smash and grab result from Denmark, which defied the odds and now has three points to give its hopes of making the Round of 16 an undeniable boost.
With first choice left back Erik Pieters unavailable, Bert van Marwijk began with Jetro Willems at the back who, at 18 years and 71 days old, became the youngest player ever to feature in the tournament.
It was the PSV Eindhoven youngster who had the first chance of the match, sending a crashing drive from long range over the bar with only three minutes gone.
Moments later, Van Persie spurned a more obvious opportunity when he belted a deflected cross off target from close range.
The Dutch appeared to be in control of proceedings, Ibrahim Afellay sending another effort off target, before Arjen Robben’s dangerous center was toe-poked into the grateful arms of Stephan Andersen.
However, for all its dominance, Netherlands lacked a cutting edge in front of goal and with 24 minutes gone, Denmark showed the team how it should be done.
Krohn-Dehli still had work to do after picking up possession just outside the area, but the Brondby winger did not hesitate, nipping past his marker and drilling between the legs of Maarten Stekelenburg.
The goal sparked Denmark into life and the team began to cause more problems by using its wide players to apply pressure down the flanks and pinging crosses into the heart of the Dutch penalty area.
The goal was almost pegged back after 36 minutes though, after a poor clearance from Andersen was pounced upon by Robben, who strode forward and bent an effort onto the base of the post.
Afellay skied another attempt following a marauding run into the box, but it was Van Persie’s miss a few minutes before halftime that left Van Marwijk shaking his head.
The Arsenal striker’s heavy first-touch betrayed a glorious set up by Wesley Sneijder, but he still should have hit the target, instead of firing wide via a deflection by Andersen.
The interval did little to turn Van Persie’s fortunes. He wasted two setups from Sneijder, with a trip over his own feet a particular cause of frustration.
Mark Van Bommel called Andersen into action with a long range pile driver, before Afellay squeezed an attempt from the edge of the box inches wide of the post, as Netherlands seemed capable of everything but putting the ball into the back of the net.
Robben planted a header the wrong side of the post, but Netherlands could have been further behind on 70 minutes had Stekelenburg not gotten a touch onto a stinging drive by Krohn-Dehli.
Denmark was holding firm at the back, and survived a late flicked header from Van Persie that inched over the bar. When a late penalty appeal for handball against Jacobsen went unanswered, the Dutch knew that their time was up.
Netherlands will have a difficult task getting its campaign back on track when it faces fellow favorite Germany in the next group match, while Denmark squares off with Portugal. Both fixtures take place next Wednesday.
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