Jorge Jesus' men were routed by PSG in their last Group C game and will have to be very wary of striker Kostas Mitroglou, who scored a hat trick against Anderlecht last time out.
Benfica will look to bounce back from a drubbing at the hands of Paris Saint-Germain when the club hosts Olympiakos in the Champions League on Wednesday.
The Group C clash in Lisbon gives the Eagles the perfect chance to respond to a disappointing 3-0 defeat in their previous European fixture.
Jorge Jesus’ men, beaten finalists in the Europa League last season, were humbled in the French capital.
Zlatan Ibrahimovic scored twice in the first 30 minutes, while Marquinhos also scored an early goal as the Ligue 1 champion cruised to victory.
Benfica is unlikely to be the only side taught a lesson by PSG this season, but the manner of the defeat will have dented the club's pride.
The Portuguese outfit opened its Champions League campaign with a 2-0 home win over Anderlecht and will hope to translate some of its recent league form into Europe's premier club competition.
After a slow start, Benfica has won three of its last four league fixtures and climbed to third in the Portuguese top flight.
However, strikers Miralej Sulejmani and Lazar Markovic are both struggling with thigh problems and Jesus has other injury issues to contend with.
Rodrigo has missed plenty of football, while fellow knee injury victim Eduardo Salvio is not expected to return in 2013.
Olympiakos followed up a 4-1 home Champions League loss to PSG with a convincing 3-0 win at Anderlecht, as Kostas Mitroglou netted a memorable hat trick.
Incredibly, Mitroglou has scored three times in four different games this season and boasts 13 goals for his club in all competitions.
He was on target in a 4-1 win Greek Super League win at Platanias on Saturday, a result that saw Olympiakos remain top of the table.
Mitroglou is now third in Olympiakos' all-time European scoring standings, with 12 goals, and Greece coach Fernando Santos has urged the Portuguese public to take the chance to watch the in-form striker.
"Go to watch him play against Benfica," he said. "He has an uncanny ability of sensing goalscoring opportunities. It's not easy to find a player with his characteristics."
Olympiakos has gone 27 games without a draw in European competition, a run dating back to August 2009, while goalkeeper Roberto has saved a penalty in each of two group fixtures this season.
Gaetan Bong (groin), Holebas (quadriceps) and Machado (knee) are all likely to miss the trip to Portugal, but Olympiakos can take heart from its terrific league form as the club seeks to cause another upset on the road.