Wales manager Chris Coleman had claimed the 30-year-old had said he was not in the right frame of mind to represent his country.
"James explained to me that he didn't want to come into the squad after being on stand-by because psychologically he was disappointed," Coleman said following his side's 3-0 defeat.
|WEST HAM LATEST
|33/1||James Collins is 33/1 with Coral to score the first goal against Southampton
Collins has since claimed that while he had a discussion with Coleman regarding his omission from the initial squad, he was never asked to join his team-mates.
"Following reports in the media today, I feel obliged to set the record straight as it has been wrongly suggested that I refused to play for my country against Serbia on the 10th Sept 2013," read a statement from Collins.
"I would like to clarify that I was not contacted by Chris Coleman or anyone at the FAW to request that I join up with the squad for the game
"Had I been asked I would have gladly joined up with my team-mates and represented my country.
"I did have a conversation with Chris to try to seek clarification on why I had been left out of the initial squad for both the Macedonia and the Serbia matches. But at no stage did I state that I would refuse to play for Wales.
"I am proud to come from Wales and even prouder to pull on the red shirt of my country so the suggestion that I would ever refuse to play for them is plainly wrong."