The former Crystal Palace and Blackpool boss was appointed earlier this week takes the struggling Lions to Huddersfield Town to look for just a third win in 11 games
Holloway was appointed as Millwall's new boss on Tuesday, signing a two-and-a-half-year deal to replace Steve Lomas.
The 50-year-old walked out at Crystal Palace in October, despite winning the club promotion to the Premier League, but will face a tougher ask at The Den, as Millwall aim to avoid relegation.
"It's a tough division but I look around that dressing room and there's some fantastic talent," Holloway told Lions Player in the build-up to the match.
"Hopefully we can stop losing – that's the first thing. I'll try and galvanise them into a way of playing – a Millwall way of hard work and effort. I want them to enjoy their football and start getting some results under their belts."
Millwall lie 21st in the table and have won just two of their last 16 matches. The London club meet a Huddersfield side who sit 13th and have scored 10 goals in their last three matches.
An intriguing encounter takes place at the King Power Stadium on Friday, as table-toppers Leicester City take on Derby County.
Derby sit fourth but have won just one of their last four matches and will be keen to bounce back with a result.
Leicester are five points clear in the automatic promotion places and have won their last four Championship fixtures.
Leeds United will hope to bounce back from a terrible 2-0 FA Cup loss at fourth-tier Rochdale when they go to Hillsborough for a Yorkshire derby against Sheffield Wednesday.
Leeds, who have not won in five matches, still sit eighth in the table, but were beaten by Rochdale and manager Brian McDermott will demand an immediate response.
Wednesday have lost just one of their last six matches, climbing out of the relegation zone and into 20th.
Rock-bottom Barnsley visit fellow strugglers Charlton Athletic, while other sides in the bottom three – Doncaster Rovers and Yeovil Town – face Blackburn Rovers and Burnley respectively.
Burnley are second in the table, four points below Leicester.
QPR sit third and visit Ipswich Town on Saturday, while other matches on the same day see promotion-hopefuls Nottingham Forest go to Bolton Wanderers and Brighton and Hove Albion meet Birmingham City.
Blackpool will look to arrest a horrendous run of form, which has seen them lose seven of their last eight, when they host an in-form Middlesbrough, while Watford face Reading and Bournemouth go to Wigan Athletic.