Pirates and Chiefs will both visit the two promoted sides, MP Black Aces and Polokwane City, in their opening games
The 2013 Telkom Knockout Cup draw was conducted on Monday night with all the big three avoiding each other in the first round.
Celtic beat Sundowns with a controversial goal to claim their first piece of silverware in many years, and revenge is looming already.
Kaizer Chiefs will visit the newly promoted boys, Polokwane City, who have not won a single game in the elite league, and are clearly in danger of getting back to the NFD.
Orlando Pirates will travel to Mpumalanga to take on Black Aces, in what is expected to be a tricky encounter for the Buccaneers.
Bloemfontein Celtic v Mamelodi Sundowns
SuperSport United v Ajax Cape Town
Free State Stars v AmaZulu
Bidvest Wits v Golden Arrows
Platinum Stars v Maritzburg United
MP Black Aces v Orlando Pirates
Polokwane City v Kaizer Chiefs
Moroka Swallows v University of Pretoria
The dates and venues for the last 16 of the tournament will be confirmed at the later stage.