The defender was part of the Dortmund side which lost 2-1 to rivals Bayern Munich in the final at Wembley in May following a memorable run to the final that included a dramatic quarter-final finish against Malaga and victory over Real Madrid in the last four.
Subotic is confident that BVB can improve on last year's showing and win the competition next term, despite the side's relative youth.
"We want to get back into the final again, and this time we don't want to lose it," he told Kicker.
"We really have a taste for it because we looked really good at the highest level. We are still young, but we don't want to waste any time."
Dortmund lost attacking midfielder Mario Gotze to Bayern earlier this summer for €37 million but have added the likes of Pierre-Emerick Aubameyang and Henrikh Mkhitaryan to their ranks, which leads Subotic to believe the team have not been weakened.
"Of course Mario is a fantastic player, but we showed while he was injured last year that we can play well without him," the 24-year-old added.
"We covered for him well and we're definitely going to be able to do that again, I can guarantee it."