The singer has been handed the No.28 shirt at the Keepmoat Stadium for next season with the club hoping he will make an appearance for the Championship side
The club have signed the 21-year-old for the 2013-14 season and he has been handed the No. 28 shirt.
Doncaster hope Tomlinson will make an appearance for the club this season with the aim of raising money for the Bluebell Wood Children's Hospice.
Tomlinson, who is an avid Doncaster fan, says he is thrilled to be signing for the Championship side.
"This has always been a childhood dream for me," he told the club's official website.
"I feel very honoured to have been asked to sign for Rovers and being able to help both the club and the amazing charity Bluebell Wood is what it’s all about."
Chairman John Ryan, meanwhile, explained the reasoning behind allowing Tomlinson to wear the No. 28 shirt.
"I am honoured to let Louis play in my number to help raise money for the charity," he said.
"I wore this in 2003 for my record-beating appearance at Hereford and this is the first time I have allowed it to be used since but I felt this was a good cause."
The move follows on from last season when Tomlinson appeared at a charity match at the Keepmoat Stadium Doncaster with the club eager to continue their fund-raising efforts.
"Louis and I organised last season’s charity game, in aid Bluebell Wood Children’s Hospice, which was a big success with over 9000 fans attending," said media manager Steve Uttley.
"It was natural to follow on from that and Louis wanted more involvement to raise the profile of his home town club and Bluebell Wood.
"What started out as light hearted banter on text has moved on to reality, which is a great coup for the club. Our next step is to get Louis on the pitch again in a Rovers kit."