The 25-year-old playmaker made a 30-minute cameo in Germany's 1-1 draw with Italy after recovering from illness, but Joachim Low has now decided to rotate his squad
The 25-year-old, who made an appearance off the bench against Italy on Friday, has been withdrawn from the squad, along with Philipp Lahm and Manuel Neuer, as Joachim Low plans to try out different players at Wembley.
"The game against England is the last chance to test tactics and personnel before the World Cup," the Mannschaft boss said in a statement.
"Lahm, Neuer and Ozil are the absolute core, for me it's important to give other players in key positions a chance to prove themselves against a great team like England."
Ozil has recorded the joint-most assists in the Premier League since moving to the Emirates Stadium during the summer, helping Arsenal climb to top spot, but was struck by illness this week.
Meanwhile, Arsenal defender Per Mertesacker joins the squad after recovering from a virus suffered prior to the 1-0 defeat to Manchester United last weekend.