The 32-year-old and South American will be among the substitutes with Pep Guardiola set to occupy the Camp Nou dugout for the final time before taking time away from the game
Xavi only makes the bench for the Blaugrana having picked up a calf problem in the 2-2 draw with Chelsea in the Champions League last month.
Jose Pinto continues in goal while Martin Montoya is selected ahead of Daniel Alves at right-back. Alexis Sanchez was ruled out of the derby in midweek with a hamstring injury while Isaac Cuenca is not risked.
Kalu Uche does not make the starting line-up for Espanyol who are also without Sergio Garcia, Felipe Mattioni and Juan Albin.
Barcelona (4-3-3): Pinto; Montoya, Puyol, Mascherano, Adriano; Thiago, Busquets, Keita; Pedro, Messi, Iniesta
Subs: Valdes, Dani Alves, Fabregas, Afellay, Xavi, Barta, Tello
Espanyol (4-2-3-1): Alvarez; Lopez, Rodriguez, Moreno, Didac; Baena, Forlin; Sanchez, Verdu, Weiss; Alvaro
Subs: Casilla, Amat, Fonte, Uche, Pandiani, Coutinho, Gomez