The Brazil international was introduced as a first-half substitute in the 2-0 Capital One Cup victory over Swindon, scoring his side's second goal with a sublime dinked finish having replaced the injured Marco van Ginkel in the 10th minute.
|14/1||Chelsea are 14/1 with Coral to beat Tottenham 2-0
"We've got an injury to Marco which is a little bit too early to be certain but it doesn't look good - he'll have a scan tomorrow on the injury to his knee and his substitute Ramires had to go off with a muscular injury at the top of the hip in the gluteal area which was causing more discomfort as he tried to run it off," he told reporters.
"He'll be a major doubt for the Tottenham game at the weekend. They're the two major disappointments of the evening."
Much of the pre-match focus was on Juan Mata, with the out-of-favour playmaker given the chance to impress from the start against League One Swindon having thus far played a bit-part role at the start of the campaign.
Holland was keen to stress that all of Jose Mourinho's attacking talents have been told they need to deliver this season, not just the Spaniard, and that he was pleased with his performance at the County Ground.
He added: "If you look at his performance tonight, he made Fernando's [Torres] chance early in the game, he was involved in the goal, so if you take those moments, it's goal-making and goalscoring actions, and then in injury time he conceded the final corner of the game supporting Ryan Bertrand in the left-back spot, which is how he was all night.
"That's been the message from Jose from day one to all our attacking players, not just Juan Mata. Jose has made it quite clear what he wants the attacking players to do in all those positions.
"That message is not just to Juan, it's to Willian, Kevin [De Bruyne], Andre Schurrle, all those players. Tonight we are very pleased with Juan's contribution, with and without the ball."