The 22-year-old right-back was at fault for the final goal in Spurs' 4-2 defeat to Chelsea on Saturday and checked out of the social network site after being insulted
The 22-year-old England international struggled to contain Juan Mata in the thrilling encounter and was badly at fault for Chelsea's fourth goal, being dispossessed by the Spaniard, who supplied the assist for Daniel Sturridge.
And Walker was left so incensed by angry messages on the social networking site that he decided to leave - though not before leaving messages in response to the abuse that he had received.
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The right-back argued that his error in losing the ball should have been mitigated by being given a free kick, continuing: "If I said what I thought to these people I would get done humans make mistakes it was 90mins and I though[t] a foul end off[sic]!!!!"
The Tottenham player won the PFA Young Player of the Year award for his performances in the 2011-12 season and has broken into the national set-up as Roy Hodgson looks to add youth and pace to his England side.
This is the latest in a long line of controversies involving footballers and Twitter, the most recent case being Ashley Cole receiving a £90,000 fine for labelling the FA a "BUNCHOFT***S" for doubting his evidence during their investigation of John Terry's use of racist language towards Anton Ferdinand.