De Guzman was included in the Dutch's final 23-man World Cup squad, confirmed on Saturday, but his place now looks less assured after it emerged on Monday that he had pulled up with an injury in training.
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"He felt something in his hamstrings and therefore I stopped the training session as we were playing 11 v 11," he told reporters.
"Now we have to wait and see in the coming days whether it is bad or not so bad.
"Hopefully it's a light injury. We will wait a couple of days and then we will be able to say what it is.
"De Guzman has earned his position in the team. He had a rather good game against Ghana on Saturday. I liked him. I said it to him, so now I have to adapt and play another player in his position, which is always not so good."
The Netherlands have been drawn in Group B alongside current world and European champions Spain, Chile and Australia.
Their first game will be against Spain in Salvador on June 13 before they face Australia in Porto Alegre on June 18 and Chile in Sao Paulo on June 23.