The Azzurri kick off their Euro 2012 campaign against the reigning world and European champions in Gdansk on Sunday evening and the former Fiorentina coach thinks his troops could learn a thing or two from Vicente Del Bosque's side.
"We still take it all too seriously on the pitch," Prandelli told reporters at his pre-game press conference. "The Spanish are more joyful; they are happy just to be playing football."
Italy's preparations have been overshadowed by the Scommessopoli scandal, while a resounding 3-0 defeat by Russia in their final warm-up fixture has significantly lowered expectation levels among the country's supporters.
However, Prandelli is confident that his team can upset the Spaniards: "I have a good feeling about this. We trained well and prepared for this match in the best possible way.
"The pessimism around us is not a problem, as, in fact, the team is more motivated to subvert it."
Italy's meeting with Spain, who knocked them out of Euro 2008 on penalties, is scheduled to kick off at 18:00CET.