The Spaniard wants the fans to act as his side's 12th man when they face Liga and city rivals Atletico Madrid in this Saturday's Champions League final in Lisbon
Carlo Ancelotti's side have not reached the showpiece of Europe's biggest competition since 2002, when they beat Bayer Leverkusen to claim the cup for the ninth time.
Isco wants the fans to back their team until the final seconds when they face Atletico Madrid on Saturday and says that it is unacceptable that the club have gone so long without winning European football's top prize.
"There's a great buzz around the team. We can't wait for Saturday - we're really up for it," he told the club's official website.
"The fans will need to act as our extra man. We really notiiced their presence from the get go in the final of the Copa del Rey.
"Hopefully we'll win La Decima. Real Madrid can't go this long without winning the final. There is one match left and we've been working towards it since July and we have to give our all.
"We have been a strong, compact and united team this season in the big games. That will key on Saturday. If we play with that desire, we have a great chance of winning.
"Atletico have been spectacular this season. We have to match them in terms of intensity. Everyone dreams of playing in a Champions League final. We will give it our all to win La Decima."