The 38-year-old said he doesn't understand what the club president meant when he said elderly people could handle the San Siro dressing room
The Dutchman took over at the helm of the Rossoneri in January this year following the sacking of Massimiliano Allegri and has climbed up the Serie A table with them as they look to secure a Europa League spot.
However, Berlusconi mocked the fledgling coach's management style, insisting that old people suffering dementia would be able to control the Milan dressing room, and Seedorf hit back at the former Italy prime minister in his press conference.
"I have nothing to say, I did not understand what he said," he told reporters. "I'm just thinking about the team and the players.
"I could joke and say that it is easy to manage this group. I'm glad that some players publicly said they are close to me, if they said it, it means they mean it.
"I don’t want to talk about the president with you, I am relaxed. He praised Vincenzo Montella? You shouldn’t ask me, he’s the one who said it. You're talking to a brick wall asking me.
"I don't want to be teased, I've already answered these questions. I don't want to be treated like a fool, I want respect.
"I know I've been working as a coach for three months, but I've been in the world of football for 22 years and I'm not stupid. I've always been respectful to the media and I want journalists to be respectful to me."
The former Milan player then turned his attention to Sunday's meeting with Atalanta and hopes his side will keep up the same intensity which has seen them win six of their last seven games, including last week's derby win over Inter.
"We’re riding the wave of the derby. Atalanta did well against Juventus and have a few young players who want to prove themselves.
"My team is in good shape and we know what is at stake here. We must always keep up that psychological balance. I want the same mentality and grit that we’ve seen in recent games."