The Chelsea manager reacted angrily to late Ramires' red card against Aston Villa last time out, encroaching on the field of play to confront referee Chris Foy
The Portuguese was sent to the stands last Saturday at Villa Park following an enraged response to Ramires' late red card, when he encroached onto the field of play to confront referee Chris Foy.
Ramires' dismissal was Chelsea's second of the match after Willian was given his marching orders for two yellow cards earlier in the Premier League encounter.
Mourinho had until Monday to lodge an appeal and announced his intention to do so on Friday.
"Yes, I have decided. I don't accept the charges," he said.
"I will do it [make his defence] privately, but for obvious reasons I don't accept the charges."
Mourinho's Chelsea side host Arsenal on Saturday in what will be Arsene Wenger's 1000th game in charge of the Gunners.