The Lazio forward could overtake Gerd Muller as die Mannschaft's all-time top goalscorer with a strike against la Roja
The Lazio striker is tied with the legendary Gerd Muller at the top Germany's all-time goalscoring charts on 68 goals heading into the clash, but he has not played for his country since September.
Having missed World Cup qualifiers against the Republic of Ireland and Sweden, as well as friendlies with Italy and England, the 35-year-old Klose is fit again and will be seeking a place in the record books when Germany welcome the South Americans to the Mercedes-Benz Arena.
Bastian Schweinsteiger has returned to Joachim Low's squad, as have Borussia Dortmund midfielder Kevin Grosskreutz and Arsenal forward Lukas Podolski.
Low has also given four uncapped players the chance to stake a claim for place in the World Cup squad. Hamburg's Pierre-Michel Lasogga is included after a season that has seen him find the net 12 times, while Augsburg attacking midfielder Andre Hahn has also been given the opportunity to impress.
Freiburg's Matthias Ginter is named among the defenders, along with Sampdoria's Shkodran Mustafi.
Germany have never played Chile and can expect a tough test against a team that enjoyed a fine World Cup qualification campaign.
La Roja come into the game having lost just two of their last 15 matches and have held their own against European nations in recent times, earning a 2-2 draw with Spain last September and beating England 2-0 at Wembley in November.
There are few surprises in Sampaoli's squad, which includes Barcelona forward Alexis Sanchez and Juventus duo Arturo Vidal and Mauricio Isla.
However, one shock call-up is that of Cardiff City's Gary Medel, who has been selected by Sampaoli despite only recently returning from a thigh injury. Fiorentina midfielder David Pizarro misses out, along with defender Miiko Albornoz.