Carlo Ancelotti's side begin their assault on another European crown with a potentially tricky trip to Turkey on Tuesday, though they will be without Xabi Alonso
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Madrid will have to do without midfielder Xabi Alonso, who remains on the sidelines with a broken foot, while Ancelotti has confirmed that Iker Casillas will start his first game of the season having been displaced by Diego Lopez in La Liga.
Galatasaray ended their 12-match winless streak against Spanish opposition when they played Madrid in the quarter-finals last season, as they recorded a 3-2 home victory that ultimately proved in vain as Jose Mourinho's men progressed 5-3 on aggregate.
With Cristiano Ronaldo on a run of 21 goals in his previous 19 Champions League matches, the Turkish side face a tough challenge in securing a repeat success.
Yet Galatasaray do boast a fine recent record in Istanbul, losing only two of their last 14 home European matches.