La Rojita will be hoping for a vast improvement to their Under-23 Olympic campaign last summer, when they were dumped out at the group stage featuring many players who have been named for the tournament in Israel in June.
Five players have been called up that were included in the victorious Spain side two years ago, including current captain David de Gea, whose performances in the tournament helped secure a move to Old Trafford in 2011.
Barca starlets Thiago, Marc Bartra and Cristian Tello will be fielded alongside Blancos youngsters such as Alvaro Morata and Nacho Fernandez.
Malaga attacker Isco will be one to watch at the Under-21 Euros, as will Athletic Bilbao talent Iker Muniain and Atletico Madrid midfielder Koke.
The lion's share of the 23-man squad ply their trade in La Liga, though the likes of Wigan goalkeeper Joel, Bayer Leverkusen star full-back Daniel Carvajal and Benfica forward Rodrigo were also named in Thursday's squad.
"The three national teams we will face in the group stage are all very competitive and contain players with a lot of experience who have featured for their seniors," Lopetegui said to reporters. "We have a wonderful challenge ahead of us."
The reigning champions qualified for the bi-yearly tournament with nine wins and a draw and have been grouped with Russia, Germany and Netherlands in the finals, which begin on June 5 and finish 13 days later.
|David de Gea||Manchester United|
|Daniel Carvajal||Bayer Leverkusen|
|Nacho Fernandez||Real Madrid|
|Inigo Martinez||Real Sociedad|
|Asier Illarramendi||Real Sociedad|
|Alvaro Morata||Real Madrid|
|Iker Muniain||Athletic Bilbao|