Mexico begin life without Jose Manuel De La Torre when they take on the USA on Tuesday.
The coach was sacked following his side's shock 2-1 home defeat to Honduras on Friday, a result that left the Central American outfit in fourth place in the CONCACAF qualifying section for Brazil 2014.
Luis Fernando Tena will take charge for the clash with Jurgen Klinsmann's side, who will be out to bounce back after being knocked off top spot following a 3-1 loss to Costa Rica.
Clint Dempsey scored a first-half penalty for the US in San Jose on Friday, but the hosts seized the initiative in the group thanks to goals from Jhonny Acosta, Celso Borges and Joel Campbell.
And Klinsmann will have to try and inspire his players to respond to this disappointment without a number of his key men.
Jozy Altidore, Matt Besler and Geoff Cameron are all suspended, while Roma midfielder Michael Bradley also misses out after picking up an ankle injury while warming-up for the game against Costa Rica.
Still, the US - who reached the last 16 of the World Cup in 2010 - will be confident of taking maximum points in Columbus, with Mexico winless in the last three meetings between the teams.