The Court of Arbitration for Sport (CAS) has confirmed that Malaga's ban from European competition has been upheld.
It was announced earlier this season that the Andalusians would face a two-year ban from the Champions League and Europa League after failing to reach financial requirements.
They vowed to fight the decision and later had the sentenced reduced to just one season, but have failed in a bid to have that overturned.
“The (CAS) has dismissed the appeal filed by Malaga against the decision taken on 21 December 2012 by the Adjudicatory Chamber of the Uefa Club Financial Control Body," a CAS statement read.
“As a consequence, the Spanish club are excluded from the 2013-14 Europa League and will have to pay a fine or €300,000.”
Malaga’s finished sixth in La Liga last term, but seventh-placed Rayo Vallecano are awaiting a CAS decision of their own concerning a Uefa license, so Sevilla could take their spot in Europe.