The Brazilian playmaker and former Wigan winger have been named among the starting eleven as both John Terry and Eden Hazard are left on the bench
Former Sao Paulo man Oscar netted twice for the Blues in their 2-2 draw with Italian outfit Juventus two weeks ago and will hope to continue that form against the Scandinavian side.
Di Matteo hands 21-year-old Moses his first European start since joining from Wigan Athletic in the summer. The forward netted his first goal for the club in the 6-0 win over Championship side Wolves in the League Cup and also played a part in the recent league win over Arsenal.
Fernando Torres is also named in the starting line-up but John Terry and Eden Hazard have both been rested with a view to this weekend's Premier League clash with Norwich.
Danish Superliga champions Nordsjaelland name an unchanged side to the team that lost 2-0 to Shakhtar Donetsk.
FC Nordsjaelland: Hansen, Okore, Mtiliga, Parkhurst, Runje, Adu, Stokholm, Lorentzen, Beckmann, Nordstrand, John
Subs: Villadsen, Gundelach, Christensen, Tičinović, Petry, Christiansen, Laudrup.
Chelsea: Cech, Ivanovic, Cole, Luiz, Cahill, Ramires, Lampard, Oscar, Torres, Mata, Moses
Subs: Turnbull, Terry, Azpilicueta, Bertrand, Romeu, Mikel, Hazard.