The Paraguay international has been on the sidelines after tearing a hamstring in the summer at the Copa America, but after a brief appearance in the Bundesliga at the weekend, could feature at the Stade Velodrome.
And the 26-year-old can't wait to play in Europe's premier club competition on Wednesday.
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"My son Tomas is as excited as I am," Barrios told Bild.
"My childhood dream was always to play in the Champions League. Hopefully, I will achieve that in Marseille," he stated.
With the South American set to return, and Ivan Perisic the hero in the 1-1 draw in the first group game against Arsenal, Kevin Grosskreutz may miss out on a starting spot.
However, the 23-year-old is ready to battle for his place in Jurgen Klopp's team.
"I will not let my regular place be taken away. Otherwise, I would be in the wrong profession. I am a fighter. Anyone who knows me knows that," Grosskreutz said.
Dortmund have started their campaign slowly, but picked up a morale boosting 2-1 win against Mainz domestically on Saturday, and Grosskreutz wants three points in France.
"We want to set off a firework in Marseille, to play aggressively and take the momentum from the Mainz game," he concluded.
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