The French left-back had not played since mid-March after the discovery of a tumour on his liver. The 31-year-old subsequently underwent surgery to have it removed and was included in the Champions League squad against Madrid by coach Pep Guardiola.
Abidal came on as a substitute for fellow defender Carles Puyol in the first minute of injury time in Barca’s 1-1 draw with their arch-rivals in the second-leg of their semi-final tie. His return to the pitch at Camp Nou was welcomed loudly by the Catalan club’s supporters.
Barcelona won the tie 3-1 on aggregate after having defeated Madrid 2-0 in the first-leg at the Bernabeu last week.