Jose Mourinho has decided to make nine changes for Madrid's fixture with Valencia with Ronaldo the biggest name on the bench
The club are to start Karim Benzema and Gonzalo Higuain up-front with support on the flanks coming from Kaka and Sergio Canales.
The injured Sami Khedira is replaced by Lassana Diarra while Esteban Granero comes in for Xabi Alonso to give the side a fresh-look in midfield.
Mourinho will have one eye on Wednesday's first-leg Champions League semi-final with Barcelona as debutant Nacho Fernandez, Ezequiel Garay and Raul Albiol come into the defence with only Iker Casillas and Ricardo Carvalho keeping their places in the team.
Madrid are currently eight points behind leaders La Liga and will be looking to close the gap with victory at the Mestalla, the stadium where they gained Copa del Rey success on Wednesday evening.
Confirmed line-ups are as follows:
Valencia (4-3-3): Guaita; Miguel, Stankevicius, R.Costa, Mathieu; Topal, Banega, Maduro; Mata, P.Hernandez, Soldado
Subs: Aduriz, Albelda, Alba, Cesar, Jonas, Joaquin, Dealbert
Real Madrid (5-4-1): Casillas; Albiol, Garay, Carvalho, Nacho, Kaka, Diarra, Granero, Canales; Benzema, Higuain
Subs: Ronaldo, Xabi Alonso, Ozil, Marcelo, Adan, Pedro Leon, Juanfran