The Spaniard believes the strength in depth at Antonio Conte's disposal will more than make up for the loss of the defender when the sides clash on Sunday
Chiellini is expected to miss the clash at Juventus Stadium through injury, but Benitez feels the squad depth at Antonio Conte's disposal means the champions will not miss the Italy international.
He told reporters: "Juve have the strongest squad in the league, the absence of Chiellini doesn't change the balance.
"They have such strength in depth and Antonio Conte knows it, so they are the favourites for the Scudetto.
"Having said that, we must play in a relaxed and confident manner, as we have faith in our own qualities.
"I expect a strong performance tomorrow, as I want to see the minds and the hearts of my players.
"Juventus are very strong, which is why we need stamina and intensity as well as the desire to do well. We will try to stop them any way they can and hurt the Bianconeri."
Napoli bolstered their ranks in the week with the signing of free agent Anthony Reveillere, with the Frenchman set to provide cover for the injured Juan Zuniga and Giandomenico Mesto.
Although he remains some way short of match fitness, Benitez is confident the defender, who turns 34 this weekend, will prove a crucial addition to the squad at San Paolo.
"We had a meeting and among the players available at the moment was Reveillere," he explained. "I knew the player and gave the all-clear to continue talks.
"He can give us a great deal and we have 15 days to get him back in shape. He has quality and can play on either flank."