The Bianconeri boss thinks Saturday could go some way to deciding the league title and Champions League places in Italy with many of the top teams all set to face each other
The Bianconeri host Fiorentina while third-placed Lazio host second-placed Napoli.
"A few paths intersect tomorrow. Fiorentina are chasing a Champions League place while Lazio and Napoli are fighting us for the Scudetto, which is a big challenge." Conte told Gazzetta.it.
Juventus will be without Leonardo Bonucci who is suspended as well as Nicklas Bendtner, Claudio Marchisio, Simone Pepe and Giorgio Chiellini who are all injured.
They will be able to call upon Andrea Pirlo, Stephan Lichtsteiner and Mauricio Isla, who had picked up knocks on midweek international duty.
Conte also went on to say that despite feelings to the contrary in Florence, Juve do not feel as much of a rivalry with Fiorentina.
"I've never really felt any rivalry with Fiorentina. They feel that more in Florence and we must not let ourselves take a provincial outlook," he continued.
"Playing against us is the greatest game of anybody's life, not just for Fiorentina."