The 37-year-old trequartista, whose contract expires this summer, will skipper his beloved Bianconeri one last time in Serie A before departing Turin for pastures new
The World Cup winner, who is out of contract this summer, will line up alongside Emanuele Giaccherini and Marco Borriello in a three-pronged Bianconeri attack.
In total, coach Antonio Conte has made seven changes in personnel to the side which clinched the Serie A title with a 2-0 win over Cagliari last weekend.
As well as the completely revamped forward line, Luca Marrone and Simone Padoin are afforded rare opportunities to shine alongside Andrea Pirlo in midfield, with Claudio Marchisio and Arturo Vidal the men to make way.
Stephan Lichsteiner, Giorgio Chiellini and Leonardo Bonucci continue in defence but they will be joined by
Marcelo Estigarribia, who will play at left-back, while Marco Storari takes over from Gianluigi Buffon in goal.
As for Atalanta, goalkeeper Andrea Consigli, versatile defender Daniele Capelli, midfielder Matteo Brighi and forward duo Guido Marilungo all miss out through injury, while centre-half Guglielmo Stendardo is suspended.
Juventus (4-3-3): Storari; Lichtsteiner, Bonucci, Chiellini, Estigarribia; Marrone, Pirlo, Padoin; Giaccherini, Borriello, Del Piero.
Subs: Manninger, Barzagli, Marchisio, Pepe, Vucinic, Matri, Quagliarella.
Atalanta (4-4-1-1): Frezzolini; Bellini, Lucchini, Manfredini, Peluso; Schelotto, Carmona, Cazzola, Bonaventura; Moralez; Denis.
Subs: Polito, Ferri, Raimondi, Cigarini, Minotti, Gabbiadini, Tribocchi.