The former Udinese man has got the nod ahead of Martin Caceres and Stephan Lichtsteiner for the right wing berth as Bianconeri boss Antonio Conte makes two changes to his lineup
Pepe takes over from Martin Caceres on the right-hand side of the Bianconeri's five-man midfield, with Stephan Lichtsteiner forced to make do with a seat on the bench, while Paolo De Ceglie replaces Alejandro Estigarribia on the opposite flank.
Elsewhere, Fabio Quagliarella, who has hit two goals in his last two appearances for the Bianconeri, will continue up front alongside Mirko Vucinic.
Lazio, meanwhile, have opted to drop the out-of-form Hernanes to the bench as Edy Reja elects to play Stefano Mauri, Alvaro Gonzalez and Antonio Candreva in behind lone striker Tommaso Rocchi.
Hernanes' fellow Brazilian Matuzalem, who is not 100 per cent fit, has also been named among the substitutes.
The Biancocelesti have arrived in Turin without a host of first-team regulars through injury, including Miroslav Klose, Andre Dias and Stefan Radu.
Juventus: Buffon; Barzagli, Bonucci, Chiellini; Pepe, Vidal, Pirlo, Marchisio, De Ceglie; Quagliarella, Vucinic.
Subs: Storari, Caceres, Lichtsteiner, Giaccherini, Borriello, Matri, Del Piero.
Lazio: Marchetti; Scaloni, Diakitè, Biava, Garrido; Cana, Ledesma; Gonzalez, Mauri, Candreva; Rocchi.
Subs: Bizzarri, Zauri, Matuzalem, Brocchi, Hernanes, Alfaro, Kozak.