The Biaconceri's meeting with the Rossoneri in Turin ended 2-2 on the night, but only after the visitors had forced extra-time by leading 2-1 at the end of 90 minutes.
Consequently, in the same way that Milan's 2-1 reversal at San Siro last month constituted a defeat on their record even though it was only the first leg of a two-legged tie, the fact that Antonio Conte's men were trailing when the whistle blew for the end of normal time at Juventus Arena means that their 31-game unbeaten run (32 games overall, taking into account the final round of last season) has now gone.
Juventus had shown impressive resilience during this season's undefeated run, most notably against Napoli last November, when they twice came from two goals down at the San Paolo to salvage a 3-3 draw with the Partenopei.
The Old Lady also came close to defeat in a controversial league outing against the Rossoneri at San Siro last month. Juventus fell behind to an early deflected strike from Milan midfielder Antonio Nocerino and then had a huge let-off when the officials failed to notice that a header from Sulley Muntari had travelled at least a metre over the line. The Bianconeri made the most of their lucky escape by snatching a point through substitute Alessandro Matri.
Of course, that their unbeaten run is now over will be of little concern to Juve, given they secured their place in May's Coppa Italia final at the expense of Milan courtesy of Mirko Vucinic's stunning extra-time strike.
In addition, Conte's side can still make history this season by becoming only the third team, after Perugia (1978-79) and Milan (1991-92), to go undefeated through an entire Serie A campaign.
Indeed, the Bianconeri have just 10 more rounds to negotiate, though they are about to embark on a make-or-break spell in which they will tackle Inter, Napoli, Palermo and Lazio.
Juve's primary concern will, however, simply be overhauling Milan at the top of the table because an unblemished season is no guarantee of Scudetto success, as the aforementioned Perugia discovered when they were pipped to the title by the Rossoneri 23 years ago after drawing 19 of their 34 games.
|JUVENTUS' UNBEATEN RUN 2011-12
|Parma||Home|| Serie A
|Siena||Away|| Serie A
||1-0||Lecce||Away||Serie A|| 1-0
|Bologna||Home|| Serie A
|Catania||Away|| Serie A
| AC Milan
||Home||Serie A||2-0||Roma||Home|| Coppa Italia
|Chievo||Away||Serie A||0-0||Udinese||Home||Serie A||2-1|
|Genoa||Home||Serie A||2-2||Siena||Home||Serie A||0-0|
|Fiorentina||Home||Serie A||2-1|| AC Milan
||Away|| Coppa Italia
|Inter||Away||Serie A||2-1||Parma||Away||Serie A|| 0-0
|Palermo||Home||Serie A||3-0||Catania||Home||Serie A|| 3-1
|Lazio||Away||Serie A||1-0|| AC Milan
||Away||Serie A|| 1-1
|Napoli||Away||Serie A||3-3||Chievo||Home||Serie A|| 1-1
|Cesena||Home||Serie A||2-0||Bologna||Away||Serie A|| 1-1
|Bologna||Home|| Coppa Italia
||1-1*||Genoa||Away||Serie A|| 0-0
|Roma||Away||Serie A||1-1||Fiorentina||Away||Serie A|| 5-0
|Novara||Home||Serie A||2-0|| AC Milan
||Home|| Coppa Italia