The towering defender could miss this weekend's match due to injury, while Jonathan dos Santos is also struggling with physical problems
Gerard Pique could miss Barcelona's clash with Real Madrid on Saturday after suffering a hamstring strain in his side's 1-1 Champions League draw with AC Milan.
The Spain international, who played the entirety of the Group H clash at San Siro on Tuesday, felt discomfort in his left leg towards the end of the match and has since undergone tests to diagnose the problem.
Further examination has now revealed that Pique could be a doubt for this weekend's Clasico, with Barcelona confirming on their official website that the 26-year-old will follow a specific workout regime on Thursday and Friday.
In a double blow for Barcelona, the club also confirmed that Jonathan dos Santos suffered an injury to the anterior cruciate ligament in his right knee during training on Wednesday.
It is not yet clear how long the Mexican midfielder will be sidelined, but the issue is being monitored by the club's medical staff and is set to be the subject of tests in the coming days.
Dos Santos has made just three league appearances for the Camp Nou side this season.