Inter midfielder Roberto Gagliardini has been added to Italy's squad to face Liechtenstein in a World Cup qualifier on Sunday.
Gagliardini, who was on international duty at an Under-21 training camp in Rome, has been called up as a replacement for Claudio Marchisio, who was withdrawn after 19 minutes of Wednesday's friendly win over Uruguay.
Juventus confirmed on Thursday that Marchisio has undergone tests that uncovered a mild groin strain in his right leg, leading to his departure from the national squad.
Giampiero Ventura is already without the services of Paris Saint-Germain midfielder Marco Verratti through injury, with Italy are level on points with Spain at the top of Group G after five matches.
An own goal from Jose Gimenez and strikes from Eder and Daniele De Rossi helped Italy to a comfortable 3-0 victory over Uruguay.
Gimenez fired past his own goalkeeper in rather spectacular fashion before hauling down Stephan El Shaarawy inside the penalty area late on, allowing De Rossi to score from the spot on what was night to forget for the defender.