Only captain Robbie Keane and Sean St Ledger survive from the side that were beaten 4-0 by the Iberians twelve months ago in Gdansk as the Italian tactician makes seven changes from Friday's World Cup qualifier against Faroe Islands.
Connor Sammon is handed the opportunity to impress alongside Keane in attack with both Shane Long and Jonathan Walters unavailable. The striker's Derby teammate Jeff Hendrick will make his first competitive start at the Yankee Stadium alongside James McCarthy, who himself returns to the side after suspension.
Trapattoni knows the size of the task facing his players but has urged them to use the game to showcase their talent against a star-studded Spanish outfit.
"This will be a great opportunity for these players against the world champions Spain who are one of the greatest teams in football," he said.
"I've reminded the players that when I was a young, I played against famous players which allowed me grow in confidence as a player.
"It will be important that we play with good balance and to show our personality. It will be a good exam for these players. Sure, Spain will will, but it's a good exam for our players."
With La Roja using the game to finalise preparations ahead of the Confederations Cup, the Irish defence will be in for a busy evening. MLS based centre-back Darren O'Dea starts alongside Sean St Ledger who will be eager to impress any potential employers after he was released by Leicester City while Paul McShane and Stephen Kelly complete the back four.
Elsewhere, Seamus Coleman will be deployed on the right flank ahead of James McClean who has to settle for a place on the bench which will contain just five outfield players given the large amount of players who didn't travel. The game kicks off at 01:00 BST at the Yankee Stadium on the morning of Wednesday June 12.Republic of Ireland XI v Spain: Forde, McShane, St Ledger, O'Dea, Kelly; Coleman, Hendrick, McCarthy, Keogh; Keane, Sammon.