The veteran defender was nearing a return to action ahead of his side's clash against Newcastle United but suffered a setback which is currently being monitored
The Villa boss had suggested that the 33-year-old centre back, who has been out injured since the summer, was in line for a return to action against Newcastle United on Tuesday January 29, but he was not named in the match squad.
Lambert revealed that the Republic of Ireland international 'felt something' on the morning of the clash against the Magpies and that he would 'wait and see' if the problem subsided.
"It looks like he has had a setback," Lambert told the Daily Telegraph. "We will have to wait and see over the next few days whether that settles down again.
"He trained on Monday but then came in this morning (ahead of the game against Newcastle) and felt something."
Dunne has been out of action for seven months after suffering a groin problem during Euro 2012 and has since undergone a series of operations to correct the issue.