According to La Repubblica, the 54-year-old and his backroom staff completed the trek on foot to the Church of the Sacred Family in Krakow, before returning to their hotel at around 05.00CET on Friday.
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It marks the third time that Prandelli has completed the ritual, with his first coming after Italy's progression to the knockout stages of the tournament, where he walked 22 km to the monastery of the Order of Camaldola.
The head coach made another pilgrimage following Italy's quarter-final success over England, walking 11 km to the Wieliczka Monastery.
If the Azzurri defeat Spain in Sunday's final, he has pledged to visit the Bieleny Monastery, located 11 km from the Turowka hotel, along with goalkeeper Gianluigi Buffon.