The Manchester United striker appeared in his first game of the tournament against Ukraine, and netted the winner as the Three Lions secured top spot in Group D with a 1-0 victory.
Barzagli praised the qualities of Rooney, but insisted that his team will be capable of containing the 26-year-old international on Sunday.
"As a team we can stop him, we are four at the back, so an effort from the entire team is needed," the Juventus defender told reporters at the pre-match press conference.
"We know just how good Rooney is and how England are as a side. We need to be 100 per cent focused to counteract this player.
|6/1||Italy are 6/1 to beat England 1-0 at Euro 2012 with Paddy Power|
Finally, Barzagli took time to comment on the atmosphere ahead the quarter-final clash against Roy Hodgson's England.
"It is the eve of a very important game. You're a bit tense, focused, but happy to be there," he added.
"There are no games better or worse at these tournaments; you just want to be there."
England and Italy kick off at 20:45CET on Sunday.