The FA confirmed the Blues pair will not travel with the Three Lions squad to Warsaw for the World Cup 2014 qualifier on Tuesday
The statement read: "Frank Lampard and Ryan Bertrand have been ruled out of England's Fifa World Cup qualifier with Poland.
"Lampard (calf) and Bertrand (illness) did not take part in England's 5-0 win over San Marino, and will not be ready for Tuesday's game."
Manager Roy Hodgson had initially hoped Lampard would recover in time to feature in Warsaw after missing the 5-0 win over San Marino.
Bertrand caused controversy last week with his rebuttle on Twitter to the accusations he had left the England camp due to a sore throat.
The 23-year-old tweeted: "Just to clarify.... Its not a 'sore throat' Im ill. Swollen glands in my neck... And constant headache for 3 days now and freezin cold.
"Do you think a 'sorethroat' could stop me being apart of a match for my club or country #yourf***ingnuts this is what every boy dreams of."
The Stamford Bridge pair will now prepare for Chelsea's difficult trip to Tottenham next Saturday.