The South American attacker did not train with the first team and is unavailable for selection, and could be joined on the sidelines by youngster Isaac Cuenca
The Chile international, who has been sidelined with a hamstring injury, did not train with the first-team squad and will miss the Primera Division game as confirmed by the club's official website.
Isaac Cuenca meanwhile has a bruised right ankle, and will undergo a late fitness test to determine whether he will be available for selection at Camp Nou.
The club also announced that former France and Manchester United forward Eric Cantona was in attendance to watch the reigning European champions train on Friday, with the retired attacker talking with Pep Guardiola ahead of the session.
The clash with Espanyol is set to be an emotional fixture, as it will be Guardiola's last game in charge of the Blaugrana at home before he steps down as head coach.
Barcelona are set to hold a closed doors training session on Saturday morning ahead of the game.