Players including Maarten Stekelenburg, Wesley Sneijder, Rafael van der Vaart and Arjen Robben have all been left out for the match in Amsterdam.
Stekelenburg has lost his starting berth at Roma this term, while Sneijder only just left Inter for Galatasaray after he was frozen out at the Serie A giants.
Van der Vaart and Robben on the other hand have been struggling with injuries recently, but featured for their respective clubs again at the weekend.
Notable newcomers in the Oranje squad are Swansea midfielder Jonathan de Guzman and Ajax wing-back Daley Blind, while players such as Ola John, Adam Maher and Marco van Ginkel made the cut, too.
Leroy Fer, Ruben Schaken and Ricky van Wolfswinkel all made the provisional selection, but were dropped for the final squad, while Khalid Boulahrouz had to pull out due to injury and was replaced by Ron Vlaar.
The match against Italy takes place at the Amsterdam ArenA on February 6.
|Ron Vlaar||Aston Villa|
|Bruno Martins Indi||Feyenoord|
|Ricardo van Rhijn||Ajax|
|Stefan de Vrij||Feyenoord|
|Marco van Ginkel||Vitesse|
|Jonathan de Guzman||Swansea|
|Robin van Persie||Manchester United|